Tetracycline inducible system
Tightly regulated control of over-expression tetracycline inducible system often necessary tetracycline inducible system study one aspect or time tetracycline inducible system of gene function and, in transgenesis, article source help to avoid lethal effects and complications caused by ubiquitous over-expression.
We have utilized the benefits of an optimized tet-on system and a modified muscle creatine tetracycline inducible system MCK promoter to generate a skeletal muscle-specific, doxycycline Dox controlled over-expression system in transgenic mice.
A DNA construct was tetracycline inducible system in which the codon optimized reverse tetracycline transactivator rtTA was placed under control of a skeletal tetracycline inducible system version of the mouse MCK promoter. Transgenic mice containing this construct expressed rtTA almost exclusively in skeletal muscles.
These mice were crossed to a second transgenic line containing a bi-directional promoter centered tetracycline inducible system a tet responder tetracycline inducible system driving both a luciferase reporter gene and a tagged gene of interest; in this case the calpain inhibitor calpastatin. Compound hemizygous mice tetracycline inducible system high level, Dox /plavix-25-pack.html muscle-specific luciferase activity often exceeding 10,fold over non-muscle tissues of the same mouse.
Tetracycline-inducible expression systems
Western tetracycline inducible system immunocytochemical analysis demonstrated similar Dox dependent muscle-specific induction tetracycline inducible system the tagged calpastatin protein.
These findings demonstrate the effectiveness and flexibility of the tet-on system to provide a tightly regulated over-expression system in adult skeletal muscle.
The MCKrtTA transgenic lines can be combined with other transgenic responder tetracycline inducible system for skeletal muscle-specific over-expression of any target gene of interest.
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Welcome to the new website for Clontech, Takara and Cellartis products - any questions please contact webmaster takarabio. Tet-On 3G tetracycline-inducible gene expression systems are the third generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced systems with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.
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Welcome to the new website for Clontech, Takara and Cellartis products - any questions please contact webmaster takarabio. The Tet system is a conditional gene expression system where transcription is turned on or off in the presence of tetracycline or doxycycline.
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A commonly used inducible mammalian expression system is the Tetracycline-controlled Transcriptional Activation system developed in by Professors Hermann Bujard and Manfred Gossen at the University of Heidelberg 1. The activator is the tetracycline transactivator fusion protein tTA: Binding of tTA to the sequences upstream of the gene of interest increases the transcription of the gene.
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